Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 13th World Congress on Biofuels and Bioenergy Mercure Hotel Wien Westbahnhof, Vienna, Austria.

Day 1 :

Keynote Forum

Christophe Len

Chimie ParisTech, France

Keynote: Catalysis for the production of furanic derivatives

Time : 10:05-10:45

OMICS International Biofuels Congress 2019 International Conference Keynote Speaker Christophe Len photo
Biography:

Christophe Len is currently a Professor at the Université de Technologie de Compiègne – UTC (France) and he develops his research at Chimie ParisTech, France. His current research explores organic chemistry and catalysis applied to biomass. He has published 170+ original publications. Among recent awards and recognition to his scientific career, he was promoted Fellow of the Royal Society of Chemistry (FRSC, 2015) and was honored with the 2017 Glycerine Innovation Award sponsored by the American Cleaning Institute and the National Biodiesel Board.

Abstract:

The concepts of sustainable development, bio-economy and circular economy are increasingly being applied to the synthesizing molecules of industrial interest. Among these molecules, furfural as a platform molecule is the subject of various research approaches to improve its synthesis and productivity and also to extend its transformation for the production of molecules of interest. Due to the current momentum in promoting green chemistry for sustainable development, chemists have recently established catalytic reactions based on alternative technologies such as continuous flow. The present study showed recent break through obtained in the production of furfural, hydroxymethylfuran, methylfuran, methyl levulinate and γ-valerolactone starting from lignocellulose in the presence of homogeneous catalysts and heterogeneous catalysts using either batch process or continuous flow process. Various reaction parameters in dependence of time such as temperature, catalyst and feedstock loadings as well as solvent types have been optimized. Conception, synthesis and physico-chemical properties will be detailed. Continuous flow transformation of biomass will pave the way to green chemistry applied to the production of biofuel.

Keynote Forum

Giuliano Degrassi

International Centre for Genetic Engineering and Biotechnology, Argentina

Keynote: Enzymatic pre-treatment of biomass for improvement of biogas production

Time : 11:00-11:40

OMICS International Biofuels Congress 2019 International Conference Keynote Speaker Giuliano Degrassi photo
Biography:

Giuliano Degrassi has completed his PhD in 2000 from the Open University of London. He is the Coordinator of the ICGEB Outstation of Buenos Aires and Leader of the Industrial Biotechnology Group. He has published more than 30 papers in peer reviewed international journals and has been the organizer of more than 20 ICGEB courses on different aspects of biotechnology.

Abstract:

Production of biogas from biomasses and organic residues by anaerobic digestion using methanogenic bacteria is an important biotechnological process for sustainable production of biofuel. One of the limiting factors of this process is the poor conversion rate into biogas of the energy contained in the biomass. This is mainly due to the difficult metabolism of the plant cell wall components by the microbial consortium present in the digestor mainly due to the complexity of cellulose, hemicellulose and lignin. Cellulose is very abundant and its full conversion into methane would increase the efficiency of the process. Biogas production from polysaccharides and other biopolymers occurs through four steps: hydrolysis, acidogenesis, acetogenesis and methanogenesis. It is evident that the importance of a more efficient hydrolysis to get more biogas produced. We developed three heterologous expression systems for production of the following enzymes like endocellulase (endo-glucanase) from Bacillus pumilus; cellobiohydrolase from Xanthomonas sp., beta-glucosidase from Bacillus amyloliquefaciens. These three enzymes are known to participate in the epolymerization of cellulose that occurs in three steps: (i) Cellulose polymer cleavage and oligomers formation; (ii) Removal of dimers (cellobiose) from the cellulose oligomers; (iii) Release of glucose from cellobiose dimers. The three genes encoding the above mentioned enzymes were amplified by PCR, cloned in pTOPO, sequenced to verify the correct amplification, then cloned in pQE, an expression vector giving 6x His tagged proteins. E. coli M15 was the expression system. The three enzymes were then purified by a single step-affinity chromatography, thanks to the six histidine tag and used in the experiments of cellulose digestion. Considering that two enzymes were not soluble when expressed in E. coli (cellobiohydrolase and beta-glucosidase formed inclusion bodies), an alternative heterologous expression system was taken into consideration for the production of the enzymes, the yeast Pichia pastoris. The final goal of the project is the development of a pretreatment method to be used for the conversion of biomasses and industrial organic residues containing cellulose into a substrate to be fermented by methanogenic bacteria for production of biogas. While the heterologous expression in Pichia is still under development, we already have an efficient system for production of the recombinant bacterial endoglucanase. The optimal conditions for the use of this enzyme have been determined and the optimal pH is 6.0 and the optimal temperature is 400C. In these conditions, pH 6.0 and temperature of 400C, the enzyme maintained up to 50% of its activity after one week. The enzyme was tested on some substrates and was found to be able to depolymerize microfibril cellulose (Sigma), residual short fiber cellulose from paper industry, corn cob powder and corn stalk powder with a specific activity of 251, 142, 75 and 70 IU/mg respectively. The next step will be the measurement of the methanogenic potential of different cellulose-containing organic residues with and without pretreatment with the cellulolytic enzyme. Following this experiment, the economic sustainability of this process will be calculated, comparing the cost of pre-treatment and the benefit achieved in term of increased biogas production.